Microscopy:Article Title: Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism
Article Snippet: Image analysis was performed on NIH ImageJ (FIJI). .. To assess muscle fiber type composition, tibialis anterior muscle cryosections on microscope slides were incubated with a 1:300 dilution of antibodies against the following MHC subtypes purchased from Developmental Studies Hybridoma Bank; type I (clone BA-F8), MHC type IIa (clone 2F7), type IIb (clone 10F5), and rabbit anti-laminin (Abcam, ab11575; 1:200) used to stain muscle membranes. .. After overnight incubation and PBS washes, muscle sections were incubated for 1 hour at room temperature with the following secondary antibodies at 1:500 dilution (Thermo Fischer Scientific, Invitrogen); Alexa Fluor 568 goat anti-mouse IgG1, Alexa Fluor 647 goat anti-mouse IgG2b, Alexa Fluor 488 goat anti-mouse IgM, and Alexa Fluor 647 goat anti-rabbit IgG.
Article Title: Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism.
Article Snippet: Image analysis was performed on NIH ImageJ (FIJI). .. To assess muscle fiber type composition, tibialis anterior muscle cryosections on microscope slides were incubated with a 1:300 dilution of antibodies against the following MHC subtypes purchased from Developmental Studies Hybridoma Bank; type I (clone BA-F8), MHC type IIa (clone 2F7), type IIb (clone 10F5), and rabbit anti-laminin (Abcam, ab11575; 1:200) used to stain muscle membranes. .. After overnight incubation and PBS washes, muscle sections were incubated for 1 hour at room temperature with the following secondary antibodies at 1:500 dilution (Thermo Fischer Scientific, Invitrogen); Alexa Fluor 568 goat anti-mouse IgG1, Alexa Fluor 647 goat anti-mouse IgG2b, Alexa Fluor 488 goat anti-mouse IgM, and Alexa Fluor 647 goat anti-rabbit IgG.
Incubation:Article Title: Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism
Article Snippet: Image analysis was performed on NIH ImageJ (FIJI). .. To assess muscle fiber type composition, tibialis anterior muscle cryosections on microscope slides were incubated with a 1:300 dilution of antibodies against the following MHC subtypes purchased from Developmental Studies Hybridoma Bank; type I (clone BA-F8), MHC type IIa (clone 2F7), type IIb (clone 10F5), and rabbit anti-laminin (Abcam, ab11575; 1:200) used to stain muscle membranes. .. After overnight incubation and PBS washes, muscle sections were incubated for 1 hour at room temperature with the following secondary antibodies at 1:500 dilution (Thermo Fischer Scientific, Invitrogen); Alexa Fluor 568 goat anti-mouse IgG1, Alexa Fluor 647 goat anti-mouse IgG2b, Alexa Fluor 488 goat anti-mouse IgM, and Alexa Fluor 647 goat anti-rabbit IgG.
Article Title: Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism.
Article Snippet: Image analysis was performed on NIH ImageJ (FIJI). .. To assess muscle fiber type composition, tibialis anterior muscle cryosections on microscope slides were incubated with a 1:300 dilution of antibodies against the following MHC subtypes purchased from Developmental Studies Hybridoma Bank; type I (clone BA-F8), MHC type IIa (clone 2F7), type IIb (clone 10F5), and rabbit anti-laminin (Abcam, ab11575; 1:200) used to stain muscle membranes. .. After overnight incubation and PBS washes, muscle sections were incubated for 1 hour at room temperature with the following secondary antibodies at 1:500 dilution (Thermo Fischer Scientific, Invitrogen); Alexa Fluor 568 goat anti-mouse IgG1, Alexa Fluor 647 goat anti-mouse IgG2b, Alexa Fluor 488 goat anti-mouse IgM, and Alexa Fluor 647 goat anti-rabbit IgG.
Immunopeptidomics:Article Title: Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism
Article Snippet: Image analysis was performed on NIH ImageJ (FIJI). .. To assess muscle fiber type composition, tibialis anterior muscle cryosections on microscope slides were incubated with a 1:300 dilution of antibodies against the following MHC subtypes purchased from Developmental Studies Hybridoma Bank; type I (clone BA-F8), MHC type IIa (clone 2F7), type IIb (clone 10F5), and rabbit anti-laminin (Abcam, ab11575; 1:200) used to stain muscle membranes. .. After overnight incubation and PBS washes, muscle sections were incubated for 1 hour at room temperature with the following secondary antibodies at 1:500 dilution (Thermo Fischer Scientific, Invitrogen); Alexa Fluor 568 goat anti-mouse IgG1, Alexa Fluor 647 goat anti-mouse IgG2b, Alexa Fluor 488 goat anti-mouse IgM, and Alexa Fluor 647 goat anti-rabbit IgG.
Article Title: Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism.
Article Snippet: Image analysis was performed on NIH ImageJ (FIJI). .. To assess muscle fiber type composition, tibialis anterior muscle cryosections on microscope slides were incubated with a 1:300 dilution of antibodies against the following MHC subtypes purchased from Developmental Studies Hybridoma Bank; type I (clone BA-F8), MHC type IIa (clone 2F7), type IIb (clone 10F5), and rabbit anti-laminin (Abcam, ab11575; 1:200) used to stain muscle membranes. .. After overnight incubation and PBS washes, muscle sections were incubated for 1 hour at room temperature with the following secondary antibodies at 1:500 dilution (Thermo Fischer Scientific, Invitrogen); Alexa Fluor 568 goat anti-mouse IgG1, Alexa Fluor 647 goat anti-mouse IgG2b, Alexa Fluor 488 goat anti-mouse IgM, and Alexa Fluor 647 goat anti-rabbit IgG.
Staining:Article Title: Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism
Article Snippet: Image analysis was performed on NIH ImageJ (FIJI). .. To assess muscle fiber type composition, tibialis anterior muscle cryosections on microscope slides were incubated with a 1:300 dilution of antibodies against the following MHC subtypes purchased from Developmental Studies Hybridoma Bank; type I (clone BA-F8), MHC type IIa (clone 2F7), type IIb (clone 10F5), and rabbit anti-laminin (Abcam, ab11575; 1:200) used to stain muscle membranes. .. After overnight incubation and PBS washes, muscle sections were incubated for 1 hour at room temperature with the following secondary antibodies at 1:500 dilution (Thermo Fischer Scientific, Invitrogen); Alexa Fluor 568 goat anti-mouse IgG1, Alexa Fluor 647 goat anti-mouse IgG2b, Alexa Fluor 488 goat anti-mouse IgM, and Alexa Fluor 647 goat anti-rabbit IgG.
Article Title: Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism.
Article Snippet: Image analysis was performed on NIH ImageJ (FIJI). .. To assess muscle fiber type composition, tibialis anterior muscle cryosections on microscope slides were incubated with a 1:300 dilution of antibodies against the following MHC subtypes purchased from Developmental Studies Hybridoma Bank; type I (clone BA-F8), MHC type IIa (clone 2F7), type IIb (clone 10F5), and rabbit anti-laminin (Abcam, ab11575; 1:200) used to stain muscle membranes. .. After overnight incubation and PBS washes, muscle sections were incubated for 1 hour at room temperature with the following secondary antibodies at 1:500 dilution (Thermo Fischer Scientific, Invitrogen); Alexa Fluor 568 goat anti-mouse IgG1, Alexa Fluor 647 goat anti-mouse IgG2b, Alexa Fluor 488 goat anti-mouse IgM, and Alexa Fluor 647 goat anti-rabbit IgG.
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